Vector Production Options


We can package more than 2 million AAV vectors with our combinations of capsid serotypes and variants, gene promotors and transgenes.

  • We offer 70+ Capsid Serotypes and Variants to provide optimal tropism for your target tissue and cell type
  • Our ever increasing library of 75+ Promoters offers both selective and ubiquitous expression of transgenes
  • We can package your transgene of interest, or select from the 350+ Transgenes available in our library, or choose custom cDNA synthesis/cloning upon request.

Capsid Serotype Options

AAV serotype Capsid mutations Cell tropism References
AAV1 RPE Burger et al.
AAV2 RPE, RGC Acland et al.; Guy et al.1
AAV2(444) Y444F RPE, RGC Petrs-Silva et al.1
AAV2(double) Y444F, Y730F RPE, RGC Petrs-Silva et al.2
AAV2(triple) Y444F+Y500F+Y730F RGC, PR Petrs-Silva et al.2
AAV2(quad) Y272F+Y444F+Y500F+Y730F RGC, PR, VEC Petrs-Silva et al.2; Cai et al.
AAV2(sext) Y252F+Y272F+Y444F+Y500F+Y704F+Y730F Kidney Petrs-Silva et al.2; Qi, YF et al.
AAV2(sept) Y252F+Y272F+Y444F+Y500F+Y700F+Y704F+Y730F RGC, PR, VEC Petrs-Silva et al.2; Ruan et al.
AAV2(quadY-F)+T-V Y272F+Y444F+Y500F+Y730F+T491V RGC, BP, BP, VEC Kay et al.; Scalabrino et al.; Boyd et al.2
AAV2(4pMut) Y444F+Y500F+Y730F+T491V RGC, PR Boye et al.3 Boyd et al.1
AAV5 PR, RPE, Cornea, Brain Boye et al.1; Beltran et al.; Mohan et al.; Burger et al.
AAV5(719) Y719F PR, RPE Michalakis et al.
AAV6-shh10 shh10 Muller cells Klimczak et al.
AAV6 (3p Mut) (705+731F+T492V
AAV6 (3p Mut)-532N Y705+731F+T492V+D532N
AAV6 (db S-V) S663V+S551V
AAV6 (db Y-F)+T-V Y705F+Y731F+T492V
AAV6 (S663V+T-V) S663V+T492V
AAV8 PR Allocca et al.
AAV8(447) Y447F PR Petrs-Silva et al.1
AAV8(733) Y733F PR, RPE Petrs-Silva et al.1; Pang et al.; Boye et al.2,  Deng et al.
AAV8(doubleYF) Y447F+Y733F) PR Kay et al.
AAV8(doubleYF+T-V) Y447F+Y733F+T494V) PR, Brain Kay et al.
AAV9 PR, Liver, Heart Lebherz et al.; Sarkar et al.

Promoter Options

Promoter Name Description/Loci (plasmid names) Size Target cell type
CMV Cytomegalovirus immediate early promoter ~600bps most cell types
CBA aka: CB, CAG Hybrid CMV/Chicken beta actin promoter 1720bps most cell types
smCBA aka: small CBA Truncated CBA promoter 953bps most cell types
MOPS aka: mOP, mRHO, MOPS500 Proximal murine rhodopsin promoter ~500bps Photoreceptors, primarily rods
GRK1 aka: hGRK, hRK, RK1 Human rhodopsin kinase 1 promoter 292bps Photoreceptor, rods and cones (mouse and primate)
IRBP aka: hIRBP241 Human inter-photoreceptor retinoid binding protein/Retinol-binding protein 3 241bps Photoreceptors, rods and cones (mouse and dog)
PR2.1 aka: CHOPS2053 Human red opsin promoter ~2100bps L and M cones
IRBP/GNAT2 hIRBP enhancer fused to cone transducin alpha promoter 524bps L/M and S cones
VMD2 aka: BEST1 Human vitelliform macular dystrophy/Bestrophin 1 promoter 625bps RPE
VEcadaka: VEcadherin VE-cadherin/Cadherin 5 (CDH5)/CD144 promoter 2530bps Vascular endothelial cells

Transgene Options

Name Description/Locus Species Notes Reference
hGFP “humanized” green fluorescent protein NA Zolotukhin et al. 1996
RFP Red fluorescent protein NA ––
mCherry NA Absorb/emiss: 587nm/610nm Ryals et al. 2011
CRE Cre recombinase P1 Bacteriophage Kaneko et al. 2011
BDNF Pease et al. 2009
CNTF Ciliary neurotrophic factor Mouse and rat Bok et al 2002: Pease et al. 2009
GDNF Glial cell derived neurotrophic factor human McGee et al. 2001
XIAP X-linked inhibitor of apoptosis protein/BIRC/BIRC4 human Potent anti-apoptotic agent McKinnon et al. 2002; Petrin et al. 2003
PEDF Pigment epithelium derived factor human Potent inhibitor of neo-vascularization Raisler et al. 2002
PDE6B Rod phosphodiesterase subunit beta mouse Pang et al 2008; Pang et al. 2011
RPE65 RPE-specific 65 kDa protein Human and dog Acland et al. 2001; Pang et al. 2006; Cideciyan et al. 2008
Myc_RPE65 Myc tagged hRPE65 human Recognized by anti-Myc antibody ––
GNAT1 Guanine nucleotide-binding protein G(t) subunit alpha-1/rod transducin alpha mouse Deng et al. 2009
GNAT2 cone transducin alpha mouse Alexander et al. 2007
MERTK Proto-oncogene tyrosine-protein kinase MER human Deng et al. 2012
ChR2 Channelrhodopsin-2/CHOP2 Chlamydomonas Doroudchi et al. 2011
ChR2-mCherry Channelrhodopsin-2 fused to mCherry ChR2 is functional ––
sENG Soluble Edoglin human ––

(table lists in stock options; custom cloning and DNA synthesis are available)