PACKAGE OPTIONS
We can package more than 2 million AAV vectors with our combinations of capsid serotypes and variants, gene promotors and transgenes.
- We offer 70+ Capsid Serotypes and Variants to provide optimal tropism for your target tissue and cell type
- Our ever increasing library of 75+ Promoters offers both selective and ubiquitous expression of transgenes
- We can package your transgene of interest, or select from the 350+ Transgenes available in our library, or choose custom cDNA synthesis/cloning upon request.
Capsid Serotype Options
| AAV serotype | Capsid mutations | Cell tropism | References |
|---|---|---|---|
| AAV1 | RPE | Burger et al. | |
| AAV2 | RPE, RGC | Acland et al.; Guy et al.1 | |
| AAV2(444) | Y444F | RPE, RGC | Petrs-Silva et al.1 |
| AAV2(double) | Y444F, Y730F | RPE, RGC | Petrs-Silva et al.2 |
| AAV2(triple) | Y444F+Y500F+Y730F | RGC, PR | Petrs-Silva et al.2 |
| AAV2(quad) | Y272F+Y444F+Y500F+Y730F | RGC, PR, VEC | Petrs-Silva et al.2; Cai et al. |
| AAV2(sext) | Y252F+Y272F+Y444F+Y500F+Y704F+Y730F | Kidney | Petrs-Silva et al.2; Qi, YF et al. |
| AAV2(sept) | Y252F+Y272F+Y444F+Y500F+Y700F+Y704F+Y730F | RGC, PR, VEC | Petrs-Silva et al.2; Ruan et al. |
| AAV2(quadY-F)+T-V | Y272F+Y444F+Y500F+Y730F+T491V | RGC, BP, BP, VEC | Kay et al.; Scalabrino et al.; Boyd et al.2 |
| AAV2(4pMut) | Y444F+Y500F+Y730F+T491V | RGC, PR | Boye et al.3 Boyd et al.1 |
| AAV5 | PR, RPE, Cornea, Brain | Boye et al.1; Beltran et al.; Mohan et al.; Burger et al. | |
| AAV5(719) | Y719F | PR, RPE | Michalakis et al. |
| AAV6 | |||
| AAV6-shh10 | shh10 | Muller cells | Klimczak et al. |
| AAV6 (3p Mut) | (705+731F+T492V | ||
| AAV6 (3p Mut)-532N | Y705+731F+T492V+D532N | ||
| AAV6 (db S-V) | S663V+S551V | ||
| AAV6 (db Y-F)+T-V | Y705F+Y731F+T492V | ||
| AAV6 (S663V+T-V) | S663V+T492V | ||
| AAV8 | PR | Allocca et al. | |
| AAV8(447) | Y447F | PR | Petrs-Silva et al.1 |
| AAV8(733) | Y733F | PR, RPE | Petrs-Silva et al.1; Pang et al.; Boye et al.2, Deng et al. |
| AAV8(doubleYF) | Y447F+Y733F) | PR | Kay et al. |
| AAV8(doubleYF+T-V) | Y447F+Y733F+T494V) | PR, Brain | Kay et al. |
| AAV9 | PR, Liver, Heart | Lebherz et al.; Sarkar et al. |
Promoter Options
| Promoter Name | Description/Loci (plasmid names) | Size | Target cell type |
|---|---|---|---|
| CMV | Cytomegalovirus immediate early promoter | ~600bps | most cell types |
| CBA aka: CB, CAG | Hybrid CMV/Chicken beta actin promoter | 1720bps | most cell types |
| smCBA aka: small CBA | Truncated CBA promoter | 953bps | most cell types |
| MOPS aka: mOP, mRHO, MOPS500 | Proximal murine rhodopsin promoter | ~500bps | Photoreceptors, primarily rods |
| GRK1 aka: hGRK, hRK, RK1 | Human rhodopsin kinase 1 promoter | 292bps | Photoreceptor, rods and cones (mouse and primate) |
| IRBP aka: hIRBP241 | Human inter-photoreceptor retinoid binding protein/Retinol-binding protein 3 | 241bps | Photoreceptors, rods and cones (mouse and dog) |
| PR2.1 aka: CHOPS2053 | Human red opsin promoter | ~2100bps | L and M cones |
| IRBP/GNAT2 | hIRBP enhancer fused to cone transducin alpha promoter | 524bps | L/M and S cones |
| VMD2 aka: BEST1 | Human vitelliform macular dystrophy/Bestrophin 1 promoter | 625bps | RPE |
| VEcadaka: VEcadherin | VE-cadherin/Cadherin 5 (CDH5)/CD144 promoter | 2530bps | Vascular endothelial cells |
Transgene Options
| Name | Description/Locus | Species | Notes | Reference |
|---|---|---|---|---|
| hGFP | “humanized” green fluorescent protein | NA | Zolotukhin et al. 1996 | |
| RFP | Red fluorescent protein | NA | –– | |
| mCherry | NA | Absorb/emiss: 587nm/610nm | Ryals et al. 2011 | |
| CRE | Cre recombinase | P1 Bacteriophage | Kaneko et al. 2011 | |
| BDNF | Pease et al. 2009 | |||
| CNTF | Ciliary neurotrophic factor | Mouse and rat | Bok et al 2002: Pease et al. 2009 | |
| GDNF | Glial cell derived neurotrophic factor | human | McGee et al. 2001 | |
| XIAP | X-linked inhibitor of apoptosis protein/BIRC/BIRC4 | human | Potent anti-apoptotic agent | McKinnon et al. 2002; Petrin et al. 2003 |
| PEDF | Pigment epithelium derived factor | human | Potent inhibitor of neo-vascularization | Raisler et al. 2002 |
| PDE6B | Rod phosphodiesterase subunit beta | mouse | Pang et al 2008; Pang et al. 2011 | |
| RPE65 | RPE-specific 65 kDa protein | Human and dog | Acland et al. 2001; Pang et al. 2006; Cideciyan et al. 2008 | |
| Myc_RPE65 | Myc tagged hRPE65 | human | Recognized by anti-Myc antibody | –– |
| GNAT1 | Guanine nucleotide-binding protein G(t) subunit alpha-1/rod transducin alpha | mouse | Deng et al. 2009 | |
| GNAT2 | cone transducin alpha | mouse | Alexander et al. 2007 | |
| MERTK | Proto-oncogene tyrosine-protein kinase MER | human | Deng et al. 2012 | |
| ChR2 | Channelrhodopsin-2/CHOP2 | Chlamydomonas | Doroudchi et al. 2011 | |
| ChR2-mCherry | Channelrhodopsin-2 fused to mCherry | ChR2 is functional | –– | |
| sENG | Soluble Edoglin | human | –– |
(table lists in stock options; custom cloning and DNA synthesis are available)